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KMID : 0361019950380050664
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Korean Journal of Otolaryngology - Head and Neck Surgery
1995 Volume.38 No. 5 p.664 ~ p.670
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Localization of Interleukin-1alpa, Interleukin-1beta, Interleukin-6 and Interleukin-8 in Human Aural Cholesteatoma
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Abstract
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Chronic otitis media with cholesteatoma can result in complications through bone destruction. Investigations on the mechanism of bone destruction by cholesteatoma suggested that the phenomenon could result from the interaction between various
agents
such as lysosomal enzyme, collagenase, acid hydrolase, prostaglandin, TNF, and IL-1, IL-1 produced by activated mononuclear phagocytes, epithelial cells, and endothelial cells induces increased production of collagenase in fibroblast-like cells.
Previous immunohistochemical studies on cholesteatomas suggested that IL-1 could be involved in bone destruction. IL-6 plays an important role in the maturation of osteoclast precursor cells into osteoclasts. Moreover, it has been shown that IL-6
stimulates osteoclastic bone resorption in fetal mouse metacarpal organ culture. IL-6 could be considered as involving in the morphological and clinical characteristics of cholesteatoma. IL-8 is produced by leukocytes, epithelial cells, and
fibroblasts,
and activates and induces degranulation in leukocytes. Since IL-8 production can be increased by IL-1 in the cultured keratinocytes, IL-8 could be responsible for the pathologic features of cholesteatoma. This study was aimed to investigate the
roles of
IL-1, IL-6 and IL-8 in bone destruction by observing their distribution pattern in normal and cholesteatomatous epithelium.
Authors observed the distribution pattern of IL-1α, IL-1β, IL-6 and IL-8 in 10% formalin fixed cholesteatoma, meatal, and postauricular skin after immunostaining with peroxidase antiperoxidase (PAP) method. All of the four cytokines were
positively
stained in the epithelial layer of the three different tissues. In basal layer of all three tissues, staining with IL-1α showed strong intensity, however tissues stained with IL-1β showed weak intensity. Staining with IL-6 and IL-8 showed no
different
intensity among each celll layers of cholesteatoma.
The results of this study suggest that IL-6 and IL-8, in addition to IL-1α and IL-1β, may be involved in the destructive characteristics of cholesteatoma. These cytokines may regulate the destructive process either alone or in combination.
(Korean J
Otolaryngol 38:5, 1995)
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